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Status
Public on Feb 01, 2007
Title
UCB-MSC_UCB1_rep3B
Sample type
RNA
Source name
human umbilical cord blood-derived mesenchymal stem cell population 1, replicate 3
Organism
Homo sapiens
Characteristics
Tissue: umbilical cord blood-derived mesenchymal stem/stromal cell
Biomaterial provider
Dr Biagio Saitta, Coriell Institute for Medical Research, 403 Haddon Ave, Camden, NJ 08103, USA, [email protected] http://www.coriell.org/ Coriell
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from cells in culture using the RNAeasy Mini Kit (Qiagen), according to manufacturer's protocol
Label
Biotin
Label protocol
Microarray target was prepared following the Affymetrix protocol. In brief, cDNA was synthesized from total RNA using the SuperScript II double-stranded cDNA synthesis kit (Invitrogen), followed by in vitro transcription, where labeled cRNA was generated (Enzo).
Description
none
Data processing
RMA method implemented in BioConductor
Submission date
Oct 12, 2006
Last update date
Oct 13, 2006
Contact name
Kenro Kusumi
E-mail(s)
[email protected]
Phone
480-727-8993
Fax
480-965-6899
URL
http://sols.asu.edu/faculty/kkusumi.php
Organization name
Arizona State University
Department
School of Life Sciences
Street address
PO Box 874501
City
Tempe
State/province
AZ
ZIP/Postal code
85287
Country
USA
Platform ID
GPL97
Series (1)
GSE6029
Cord Blood-Derived Mesenchymal Stem Cells with Distinct Growth Kinetics, Differentiation Potentials, Expression Profiles
Data table header descriptions
ID_REF
VALUE
RMA expression estimate
Data table
ID_REF
VALUE
200000_s_at
8.83991145057334
200001_at
10.6431993301252
200002_at
11.3026117825239
200003_s_at
11.3049134060956
200004_at
10.8646939505868
200005_at
9.79556892383023
200006_at
10.929538743157
200007_at
10.3869173244728
200008_s_at
9.29177167232945
200009_at
10.7032835078798
200010_at
11.0196002353623
200011_s_at
8.99841783130321
200012_x_at
11.6837525419825
200013_at
11.3573383959077
200014_s_at
9.96156623039462
200015_s_at
9.8138790201352
200016_x_at
11.3664405685729
200017_at
11.150812391331
200018_at
11.6432724453599
200019_s_at
10.8203897481276
Total number of rows: 22645 Table truncated, full table size 602 Kbytes .Supplementary file
Size
Download
File type/resource
GSM139903.CEL.gz
3.1 Mb
(ftp) (http)
CEL