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Status
Public on Feb 01, 2007
Title
UCB-MSC_UCB1_rep1B
Sample type
RNA
Source name
human umbilical cord blood-derived mesenchymal stem cell population 1, replicate 1
Organism
Homo sapiens Characteristics
Tissue: umbilical cord blood-derived mesenchymal stem/stromal cell
Biomaterial provider
Dr Biagio Saitta, Coriell Institute for Medical Research, 403 Haddon Ave, Camden, NJ 08103, USA, [email protected] http://www.coriell.org/
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from cells in culture using the RNAeasy Mini Kit (Qiagen), according to manufacturer's protocol
Label
Biotin
Label protocol
Microarray target was prepared following the Affymetrix protocol. In brief, cDNA was synthesized from total RNA using the SuperScript II double-stranded cDNA synthesis kit (Invitrogen), followed by in vitro transcription, where labeled cRNA was generated (Enzo).
Hybridization protocol
Hybridization was carried out according to Affymetrix protocol.
Description
none
Data processing
RMA method normalization implemented in BioConductor
Submission date
Oct 12, 2006
Last update date
Oct 13, 2006
Contact name
Kenro Kusumi
E-mail(s)
[email protected] Phone
480-727-8993
Fax
480-965-6899
URL
http://sols.asu.edu/faculty/kkusumi.php Organization name
Arizona State University
Department
School of Life Sciences
Street address
PO Box 874501
City
Tempe
State/province
AZ
ZIP/Postal code
85287
Country
USA
Platform ID
GPL97 Series (1)
GSE6029 Cord Blood-Derived Mesenchymal Stem Cells with Distinct Growth Kinetics, Differentiation Potentials, Expression Profiles
Data table header descriptions ID_REF VALUE RMA expression estimate
Data table ID_REF VALUE 200000_s_at
8.86250762968547
200001_at
10.4769114749315
200002_at
11.432385396217
200003_s_at
11.505795956061
200004_at
10.6774689599481
200005_at
10.1009189515425
200006_at
10.9825403600137
200007_at
10.4685164876302
200008_s_at
9.78533617762242
200009_at
10.712295682276
200010_at
11.0640503084056
200011_s_at
8.94099846073278
200012_x_at
11.7330833867348
200013_at
11.6773879304202
200014_s_at
9.79484980387357
200015_s_at
10.0306280021983
200016_x_at
11.225986090127
200017_at
11.2057558736453
200018_at
11.8383323620961
200019_s_at
11.2055786423156
22645 602 Kbytes .Supplementary file Size Download File type/resource GSM139896.CEL.gz
2.9 Mb
(ftp) (http) CEL
