|
| Status |
Public on May 07, 2014 |
| Title |
naive WT P14_2 |
| Sample type |
RNA |
| |
|
| Source name |
Day 3 post LCMV infection sorted P14 cells from the spleen of naive mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
nk depletion: no
genetic background: Bl6
genotype: WT
treatment: none
|
| Treatment protocol |
naïve Bl6 mice were depleted of NK cells using the aNK1.1 depleting antibody or left untreated, followed by co-transfer of WT and Ifnar1-/- P14 cells. One day later the mice were infected with LCMV8.7 + Vaccinia Virus-G2. At day 3 post infection WT and Ifnar1-/- P14 cells were FACS sorted to purity, frozen and shipped to Miltenyi Biotec for microarray analysis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using standard protocols (NucleoSpin RNA II)
|
| Label |
Cy3
|
| Label protocol |
Cy3 labelling was achieved using the Agilent low input quick Amp labeling kit (Agilent Technologies)
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit. 600ug Cy3-labeled fragmented cRNA in hybridization buffer was hybridized overnight to Agilent Whole Mouse Genome Oligo Microarrays 8x60K using Agilents recommended hybridization chamber and oven. The microarrays were washed once with the Agilent Gene Expression Wash Buffer 1 for 1 min at room temperature followed by a second wash with preheated Agilent Gene expression wash buffer 2 (37 °C) for 1 min. The last washing step was performed with acetonitrile.
|
| Scan protocol |
Flourescene signals of the hybridized Agilent Microarrays were detected using Agilent`s Microarray Scanner System.
|
| Data processing |
Agilent Feature Extraction Software was use to read out and process the microarray image files.
|
| |
|
| Submission date |
May 06, 2014 |
| Last update date |
May 07, 2014 |
| Contact name |
Annette Oxenius |
| E-mail(s) |
[email protected]
|
| Organization name |
ETH Zurich
|
| Department |
Microbiology
|
| Lab |
Oxenius
|
| Street address |
Vladimir-Prelog-weg 1-5/10
|
| City |
Zurich |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE57355 |
The effect of type-I interferon signaling directly on CD8+ T cells following LCMV infection in the presence or absence of NK cells. |
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