|
| Status |
Public on Apr 29, 2014 |
| Title |
HaCaT Obacunone-treated 3 h |
| Sample type |
RNA |
| |
|
| Source name |
HaCaT cell
|
| Organism |
Homo sapiens |
| Characteristics |
agent: 100 uM obacunone
time point: 3 h
cell type: keratinocyte
|
| Treatment protocol |
Cells were seeded at a density of 1 x 106 cells in a 100 mm dish and incubated overnight. Next day, the cells were fed with fresh media containing 10% FBS and incubated with 100 uM obacunone or 0.1% DMSO (vehicle control) for 0.5, 1, 3, 6, or 12 h.
|
| Growth protocol |
Cells were maintained in DMEM supplemented with 10% FBS and antibiotics at 37°C in a humidified 5% CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate1
SAMPLE 9
|
| Data processing |
The data were normalised using quantile normalisation with Avadis 3.3 software
|
| |
|
| Submission date |
Apr 25, 2014 |
| Last update date |
Apr 29, 2014 |
| Contact name |
Jong Min Kim |
| E-mail(s) |
[email protected]
|
| Organization name |
Sejong University
|
| Department |
Bioscience and Biotechnology
|
| Street address |
209, Neungdong-ro, Gwangjin-gu
|
| City |
Seoul |
| ZIP/Postal code |
143-747 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE57080 |
Temporal gene regulation of human keratinocyte HaCaT cells treated with obacunone |
| GSE57121 |
Gene expression profiles of human keratinocytes and hepatocytes treated with obacunone |
|
