|
| Status |
Public on Feb 13, 2017 |
| Title |
CD4+ T cells of chen meiwen T |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells from PBMC of healthy control
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: healthy control
cell type: CD4+ T cells from PBMC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A total of 12 ml whole blood samples were drawn and stored at 4℃ less than 4h. Samples were layered onto Ficoll-Paque Plus and PBMCs were separated by density gradient centrifugation. An automated magnetic beadbased positive selection protocol was used to isolate CD4 cells (Stemcell Technologies, Vancouver, Canada).Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000
|
| Description |
Gene expression data from CD4+ T cells of healthy controls
|
| Data processing |
The gene expression array data were digitalized using GeneChip Operating Software (GCOS) (v.1.4, Affymetrix), and normalized by eliminating the highest and lowest 2% of the data using MAS5 algorithm (Affymetrix).
|
| |
|
| Submission date |
Apr 09, 2014 |
| Last update date |
Feb 13, 2017 |
| Contact name |
Jianping Guo |
| Organization name |
Peking University, People's Hospital
|
| Department |
Rheumatology & Immunology
|
| Street address |
NO.11,Xizhimen South Street,Xicheng District
|
| City |
Beijing |
| ZIP/Postal code |
100044 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE56649 |
Expression data from active rheumatoid arthritis patients and healthy control. |
|
