|
| Status |
Public on Mar 27, 2014 |
| Title |
10 |
| Sample type |
RNA |
| |
|
| Source name |
dissected lung tumor
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: KrasG12D
diet: high calorie
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
high calorie diet 5
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 26, 2014 |
| Last update date |
Mar 27, 2014 |
| Contact name |
Giorgio Ramadori |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Geneva
|
| Street address |
Michel Servet 1
|
| City |
Geneva |
| ZIP/Postal code |
1227 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE56260 |
Gene expression in Kras-driven lung tumors in mice fed with standard or high calorie diet |
|
