|
| Status |
Public on Mar 19, 2014 |
| Title |
Btg1:Btg2 dKO_BM B220+ CD43+ cells |
| Sample type |
RNA |
| |
|
| Source name |
BM B220+ CD43+
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
cell type: BM B220+ CD43+
genotype: Btg1;Btg2 dKO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
BM was isolated from tibia and femur by flushing. The BM B220+ CD43+ and B220+ CD43- fraction were sorted by flow cytometry. RNA was extracted QIAGEN RNeasy kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Illumina Ambion Total Prep-96 Kit
|
| |
|
| Hybridization protocol |
Standard Illumina direct hybridization protocol
|
| Scan protocol |
Standard Illumina direct hybridization scanning protocol
|
| Description |
Hardy fractions ABC
|
| Data processing |
The data were normalised using RMA normalisation with Arraystar
|
| |
|
| Submission date |
Mar 18, 2014 |
| Last update date |
Mar 19, 2014 |
| Contact name |
Blanca Scheijen |
| E-mail(s) |
[email protected]
|
| Organization name |
Radboudumc
|
| Lab |
Pediatric Oncology
|
| Street address |
Kapittelweg 29
|
| City |
Nijmegen |
| ZIP/Postal code |
6525 EN |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL15097 |
| Series (1) |
| GSE55997 |
BTG1 and BTG2 Regulate B Linege Commitment by Modulation of EBF1 Activity |
|
