|
| Status |
Public on Sep 15, 2015 |
| Title |
cell line gemcitabine2 |
| Sample type |
RNA |
| |
|
| Source name |
human cholangiocellular carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CCC
treatment: 0.1ug/ml gemcitabine for 48hrs
|
| Treatment protocol |
CCC cells were treated with 0.1μg/ml gemcitabine or without gemcitabine for 48 h.
|
| Growth protocol |
Cells were grown in RPM I 1640(sample1～4) or DMEM (sample5～8) supplemented with 10% fetal bovine serum in a humidified atmosphere of 5% CO2 at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using miRNesy mini kit (Quiagen).
|
| Label |
Hy5
|
| Label protocol |
Extracted total RNA was labeled with Hy5 using the miRCURY LNA Array miR labeling kit (Exiqon, Vedbaek, Denmark)
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
gem-2
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals. Measurements of both duplicate spots with the signal intensities greater than 2 standard deviations (SD) of the background signal intensity were considered to be valid. A relative expression level of a given miRNA was calculated by comparing the signal intensities of the averaged valid spots with their mean value throughout the microarray experiments.
|
| |
|
| Submission date |
Mar 06, 2014 |
| Last update date |
Sep 15, 2015 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15446 |
| Series (1) |
| GSE55676 |
Study of microRNA associated with gemcitabine’s anti-tumor effect |
|
