|
| Status |
Public on Mar 07, 2014 |
| Title |
E18.5 lung WT1, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic lung at E18.5 days WT
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Embryonic lung E18.5
genotype: C57BL/6 WT
age: Embryonic Day 18.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from E18.5 lungs was isolated with RNeasy® plus mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Gene 1.0 ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Expression Console.
|
| Description |
Gene expression data from embryonic lungs at day E18.5 Wild type
Lungs of timed-pregnant C57BL/6 wild type (+/+) and Hmga2 ( -/-) were dissected from the embryos at E18.5
|
| Data processing |
Data were analyzed by the Robust Multi-array Average (RMA) algorithm using the Affymetrix Expression Console. An unpaired t test was performed with log2-transformed data to identify significantly differentially expressed transcripts. Fold change was calculated using DNAStar ArrayStar 11.0 software
|
| |
|
| Submission date |
Feb 25, 2014 |
| Last update date |
Mar 07, 2014 |
| Contact name |
Guillermo Barreto |
| E-mail(s) |
[email protected]
|
| Organization name |
CNRS Director of Research
|
| Department |
Campus Biologie-Santé. Faculté de Médecine
|
| Lab |
Laboratoire IMoPA. UMR 7365 CNRS
|
| Street address |
9, Avenue de la Forêt de Haye ‐ BP 20199
|
| City |
Nancy Cedex |
| ZIP/Postal code |
54505 |
| Country |
France |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE55340 |
Hmga2 is required for canonical WNT signaling during lung development |
|
