|
| Status |
Public on Feb 20, 2014 |
| Title |
Young8_Mouse430 |
| Sample type |
RNA |
| |
|
| Source name |
distal trachea and carina region of 2 month old mice
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57Bl/6
gender: female
age: 2 month
tissue: distal trachea and carina region
|
| Treatment protocol |
Distal tracheas and carinas of four young (2 month) and four older (14 month) C57Bl/6 female mice were dissected out.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the distal tracheas and carinas using QIAshredder and RNeasy Micro Kits (QIAGEN).
|
| Label |
Biotin
|
| Label protocol |
Total RNA was processed using Ambion MessageAmpTM Premier by the Duke Microarray Facility.
|
| |
|
| Hybridization protocol |
Affymetrix ENZO labeling kit
|
| Scan protocol |
Hybridization of samples to Affymetrix GeneChip arrays was performed according to Affymetrix manual instructions
|
| Data processing |
Scanning of Affymetrix GeneChip arrays was performed according to the Affmetrix manual instructions. Data analysis was performed using Genomics Suite 6.5 (Partek) software and robust multichip analysis normalization was performed on each dataset. Two-way ANOVA and fold-change analyses were performed to select genes that were differentially expressed between 2 month and 14 month old C57Bl/6 datasets. Top differentially expressed genes were selected with a p-value cutoff of <0.05 based on ANOVA test and a fold-change cutoff of >±2.0.
|
| |
|
| Submission date |
Feb 19, 2014 |
| Last update date |
Feb 20, 2014 |
| Contact name |
Brigid Hogan |
| E-mail(s) |
[email protected]
|
| Phone |
919-684-8085
|
| Organization name |
Duke University Medical Center
|
| Department |
Cell Biology
|
| Lab |
Hogan Lab
|
| Street address |
388 Nanaline Duke Bldg PO Box 3709
|
| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE55162 |
Age-related changes in the cellular composition and epithelial organization of the mouse trachea |
|
