|
| Status |
Public on Feb 18, 2014 |
| Title |
WT_LPS |
| Sample type |
RNA |
| |
|
| Source name |
CD11c+CD80+CD86+MHC-II+
|
| Organism |
Mus musculus |
| Characteristics |
treatment: LPS
genotype: wild type
genetic background: C56BL/6
cell type: Spleen dendritic cells
|
| Treatment protocol |
Pure dendritic cells from the spleens of WT and MARCO KO mice were cultured overnight in PBS or Lipopolysaccharide (LPS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol.
|
| Label |
Biotin
|
| Label protocol |
Biotin-UTP was incorporated during the overnight in vitro transcription step according to the manufacturer's (Ambion® | Life Technologies) protocol.
|
| |
|
| Hybridization protocol |
cRNA (15 μg) was fragmented and hybridized to arrays according to the manufacturer's (Ambion® | Life Technologies) protocols.
|
| Scan protocol |
Arrays were scanned with the GeneChip Scanner 3000 and GeneChip Operating Software acquired raw fluorescence signal.
|
| Description |
WT DC + LPS
|
| Data processing |
Scanned array images and CEL file data were analyzed and normalized using RMAexpress.
|
| |
|
| Submission date |
Feb 15, 2014 |
| Last update date |
Feb 18, 2014 |
| Contact name |
M. Simo Arredouani |
| E-mail(s) |
[email protected]
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Department |
Surgery
|
| Street address |
330 Brookline Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE55068 |
Contribution of the scavenger receptor MARCO to TLR-induced activation of dendritic cells |
|
