|
| Status |
Public on May 16, 2014 |
| Title |
Cast_2 |
| Sample type |
RNA |
| |
|
| Source name |
CD3+CD4+GFP- cells from spleens of Foxp3-GFP mice
|
| Organism |
Mus musculus |
| Characteristics |
surgery: castrated
cell type: CD3+CD4+ GFP- Splenocytes
|
| Treatment protocol |
Mice were castrated or received sham surgery.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CD3+CD4+GFP- cells isolated from the spleens of mice 4 weeks after surgery. RNA extracted using Trizol.
|
| Label |
Biotin
|
| Label protocol |
Biotin-UTP was incorporated during the overnight in vitro transcription step according to the manufacturer's (Ambion® | Life Technologies) protocol.
|
| |
|
| Hybridization protocol |
cRNA (15 μg) was fragmented and hybridized to arrays according to the manufacturer's (Ambion® | Life Technologies) protocols.
|
| Scan protocol |
Arrays were scanned with the GeneChip Scanner 3000 and GeneChip Operating Software acquired raw fluorescence signal.
|
| Data processing |
Scanned array images were analyzed by dChip and CEL file data were normalized using RMAexpress.
|
| |
|
| Submission date |
Feb 12, 2014 |
| Last update date |
May 16, 2014 |
| Contact name |
Haydn Kissick |
| E-mail(s) |
[email protected]
|
| Organization name |
Beth Israel Deaconess Medical Ceter
|
| Street address |
3 Blackfan Circle, CLS 4/430
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02116 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE54945 |
The effect of androgens on CD4 T-cells |
|
