|
| Status |
Public on Apr 14, 2014 |
| Title |
WT-3 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Embryonic Fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Rb +/+
growth condition: Serum Deprived
cell type: Mouse Embryonic Fibroblasts
|
| Treatment protocol |
N/A
|
| Growth protocol |
MEFs were plated at low density 3 million cells per 15 cm culture dish. 24h later media was replaced with media cotaining 0.1%FBS to induce quiescence
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted according to Trizol user manual followed by purification by reprecititation with sodium acetate and 100% ethanol
|
| Label |
biotin
|
| Label protocol |
Biotin end labeled single strand cDNA was prepared according to the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual
|
| |
|
| Hybridization protocol |
5.5 ug of single stranded cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
CEL files were converted to RMA expression values using BRB-Array Tools software (http://linus.nci.nih.gov/BRB-ArrayTools.html). BRB-Array Tools uses the affy package in R to calculate RMA values.
|
| |
|
| Submission date |
Feb 12, 2014 |
| Last update date |
Apr 14, 2014 |
| Contact name |
Fred Dick |
| E-mail(s) |
[email protected]
|
| Organization name |
Western University
|
| Street address |
Cancer Research Labs 790 Commissioners Road East
|
| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A 4L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE54924 |
A retinoblastoma allele that is mutated at its common E2F interaction site inhibits cell proliferation in gene targeted mice |
|
