|
| Status |
Public on Feb 12, 2014 |
| Title |
H3K9me2_Z2 Sample 6 |
| Sample type |
SRA |
| |
|
| Source name |
Salivary Glands
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: Salivary Gland Cells
developmental stage: Third instar larvae
strain: JIL-1 -/-
chip antibody: Abcam Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220]
|
| Growth protocol |
Salivary gland cells were isolated from wild type and JIL-1 null mutant 3rd instar larvae of Drosophila Melanogaster
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Libraries were prepared according to Illumina's instructions
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
ChIP-seq reads were aligned to the dm3(BDGP5.72) Drosophila genome assembly using Bowtie V0.12.7
SAMtools V0.1.18 and BEDtools V2.14.3 were used to sort and transfer files.
peaks were called using SICER V1.1 with the following setting: 200 bp window size, 200 bp gap size, and an effective genome size of 72% of the Drosophila genome
|
| |
|
| Submission date |
Jan 27, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jorgen Johansen |
| E-mail(s) |
[email protected]
|
| Phone |
5152949783
|
| Organization name |
Iowa State Universtiy
|
| Department |
BBMB
|
| Lab |
Johansen's Lab
|
| Street address |
MBB3142, Iowa State Univeristy
|
| City |
Ames |
| State/province |
Iowa |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platform ID |
GPL9061 |
| Series (2) |
| GSE41047 |
Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 kinase mediated histone H3S10 phosphorylation in drosophila |
| GSE54438 |
Genome-wide analysis of H3K9me2 and JIL-1 kinase mediated histone H3S10 phosphorylation in drosophila [ChIP-seq: 13 samples] |
|
| Relations |
| BioSample |
SAMN02597566 |
| SRA |
SRX450793 |
