|
| Status |
Public on Nov 07, 2014 |
| Title |
GM12144, cDNA |
| Sample type |
RNA |
| |
|
| Source name |
GM12144
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: Lymphoblasts
population: CEU
|
| Biomaterial provider |
http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM12144
|
| Growth protocol |
The LCLs were grown in T75 flasks in 1X RPMI 1640 Media (invitrogen, Burlington, ON), With 2 mM L-glutamine, 15% fetal bovine serum and 1% penicillin/streptomycin at 37°C with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted from cell lysates resuspended in 600 mL RLT lysis buffer using the Rneasy Mini Kit (Qiagen. DNA was extracted from cell lysates resuspended in 200 mL PBS using the GenElute DNA Miniprep Kit (SigmaAldrich).
|
| Label |
Cy3 and Cy5
|
| Label protocol |
according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
according to manufacturer's instructions
|
| Scan protocol |
according to manufacturer's instructions
|
| Description |
XC01334, paternal grandfather
|
| Data processing |
using GenomeStudio from illumina
|
| |
|
| Submission date |
Jan 27, 2014 |
| Last update date |
Nov 13, 2014 |
| Contact name |
Tomi Markku Pastinen |
| E-mail(s) |
[email protected]
|
| Organization name |
McGill University and Génome Québec Innovation Centre
|
| Street address |
740, Dr. Penfield Avenue
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A 1A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6983 |
| Series (1) |
| GSE54252 |
Allele specific gene expression in tissue |
|
