|
| Status |
Public on Dec 31, 2014 |
| Title |
Gfi1b knock-out Ter119 positive cells |
| Sample type |
RNA |
| |
|
| Source name |
Mouse bone marrow derived CD11b+, Gr1-low monocytes from Gfi1 knock-out mouse
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Ter119 positive erythroid cells
genotype/variation: Gfi1b knock-out
|
| Treatment protocol |
Six weeks old mice were treated five times with pIpC (500µg each) every other day and sacrificed 42 days after the first injection
|
| Growth protocol |
Mice were housed under Specific Pathogen Free (SPF) conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol (Invitrogen) was used to isolate total RNA following the manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Target RNA was reverse transcried into cDNA and in-vitro transcribed using the Ovation Pico WTA system (NuGEN) according to the Manufacturers protocols from 50 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix) to generate biotin-labeled cRNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 8 ug of cRNA were hybridized with Affymetrix Mouse Genome 430 2.0 Arrays and Scanned using the Afymetrix scanner M10 at the BioChip laboratory of the University Hospital of Essen, Germany
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
Gene expression data from bone marrow derived Gfi1b conditional knockout erythroid cells
|
| Data processing |
Data was processed using the AltAnalyze software (www.altanalyze.org) using the default parameters.
|
| |
|
| Submission date |
Jan 17, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Cyrus Khandanpour |
| E-mail(s) |
[email protected]
|
| Organization name |
University Hospital Of Essen
|
| Department |
Department of Hematology
|
| Lab |
WTZ-Forschungsgebäude 2.OG
|
| Street address |
Hufelandstr. 55
|
| City |
Essen |
| State/province |
NRW |
| ZIP/Postal code |
45147 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE54205 |
Growth factor independence 1b (Gfi1b) is required for erythroid cell maturation and regulates embryonic globin expression [Mouse430_2] |
| GSE54206 |
Growth factor independence 1b (Gfi1b) is required for erythroid cell maturation and regulates embryonic globin expression |
|
