|
| Status |
Public on Dec 12, 2014 |
| Title |
CDR131_sham |
| Sample type |
RNA |
| |
|
| Source name |
C57Bl/6N_Sham-operated control
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6N
gender: male
subjected to: sham operation (control)
tissue: left ventricles
|
| Treatment protocol |
Angiotensin II infusion in mini-osmotic pumps implanted sub-cutaneously, treated 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Rneasy microkit with DNase I treatment in accordance with the prescribed protocol (fibrous tissue) provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
CDR131
|
| Data processing |
quantile normalisation with package preprocessCore 1.25.3
|
| |
|
| Submission date |
Dec 12, 2013 |
| Last update date |
Dec 12, 2014 |
| Contact name |
Sophie Cardin |
| E-mail(s) |
[email protected]
|
| Phone |
514-987-5760
|
| Organization name |
IRCM
|
| Department |
Experimental medecine
|
| Lab |
CFDeschepper
|
| Street address |
110, Pine west
|
| City |
Montreal |
| State/province |
Qc |
| ZIP/Postal code |
H2W1R7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL15097 |
| Series (1) |
| GSE53269 |
Gene expression profiling in left cardiac ventricles of C57Bl/6J and C57Bl/6N male mice treated for 48 h with angiotensin II 500 ng/kg/min. |
|
