|
| Status |
Public on Nov 28, 2013 |
| Title |
Uninfected, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Uninfected, replicate 3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: THP-1
|
| Treatment protocol |
Cells were differentiated with PMA
|
| Growth protocol |
Cells were grown and infected in RPMI-1640 with 10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol-choloform extraction
|
| Label |
biotin
|
| Label protocol |
Target RNA is reverse transcribed into cDNA and in-vitro transcription is performed to generate biotin-labeled cRNA for subsequent hybridization.
|
| |
|
| Hybridization protocol |
cDNA was hybridized onto Affymetrix Human Gene 1.0 ST chips
|
| Scan protocol |
Hybridized target cRNA is stained with streptavidin phycoerythrin and arrays are scanned using a GeneArray Scanner at an excitation wavelength of 488nm
|
| Description |
Monocyte cell line terminally differentated into macrophages by PMA treatment
Gene expression data from macrophages
|
| Data processing |
Data was processed using Flexarray v1.6.1
|
| |
|
| Submission date |
Nov 27, 2013 |
| Last update date |
Sep 01, 2016 |
| Contact name |
John White |
| E-mail(s) |
[email protected]
|
| Organization name |
McGill University
|
| Department |
Physiology
|
| Street address |
3655 Prom. Sir William Osler
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3G 1Y6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE52819 |
Vitamin D treatment of M.tb. infected macrophages |
|
| Relations |
| Reanalyzed by |
GSE86357 |
