|
| Status |
Public on Feb 26, 2014 |
| Title |
KH_vs_TH |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
KH DNA
|
| Organism |
Equus caballus |
| Characteristics |
genotype: Wild type
breed: Kazakh horse
tissue: blood
|
| Treatment protocol |
Blood was directly collected from horse
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The genomic DNA was extracted using Promega Genomic DNA Purification Kit (A1620).
|
| Label |
Cy3
|
| Label protocol |
The sample labelling was using NimbleGen Dual-Color DNA Labeling Kit (05223547001).
|
| |
|
| Channel 2 |
| Source name |
TH DNA
|
| Organism |
Equus caballus |
| Characteristics |
genotype: wild type
breed: Thoroughbred
tissue: blood
|
| Treatment protocol |
Blood was directly collected from horse
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The genomic DNA was extracted using Promega Genomic DNA Purification Kit (A1620).
|
| Label |
Cy5
|
| Label protocol |
The sample labelling was using NimbleGen Dual-Color DNA Labeling Kit (05223547001).
|
| |
|
| |
| Hybridization protocol |
Hybridization was processing 60h at 42°C in Roche-NimbleGen hybridization system12.
|
| Scan protocol |
Arrays were scanned on an Roche-NimbleGen MS200 scanner.
|
| Description |
CNV analysis of KH vs Thoroughbred
|
| Data processing |
Arrays were processed using NimbleScan,which transformed image signal to digital signal, following using segMNT algorithm to analyse the normalized signal ratio.
|
| |
|
| Submission date |
Nov 19, 2013 |
| Last update date |
Feb 26, 2014 |
| Contact name |
Wei Wang |
| E-mail(s) |
[email protected]
|
| Phone |
86-15124775364
|
| Organization name |
College of Life Sciences Chinese Inner Mongolia Agricultural University
|
| Lab |
Key Laboratory of biological manufacturing
|
| Street address |
Inner Mongolia Hohhot City Saihan District Zhao Wuda road 306
|
| City |
Hohhot |
| ZIP/Postal code |
010018 |
| Country |
China |
| |
|
| Platform ID |
GPL17952 |
| Series (1) |
| GSE52504 |
Genome-wide detection by array CGH of copy number variations among diverse horse breeds |
|
