|
Status |
Public on Nov 19, 2013 |
Title |
primary_lung_cancer_B120 |
Sample type |
RNA |
|
|
Source name |
primary lung cancer
|
Organism |
Homo sapiens |
Characteristics |
tissue: primary lung cancer Post Surgical Treatment: OBS Stage: 1B age: 35.4 Sex: Male Cause of death: Alive Histology type: ADC OS time: 6.12 OS status: Alive dss survival time: 6.12 DSS status: Alive dcc sample: Yes predominant subtype: invasive mucinous
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Data processing |
The data were preprocessed using RMA v0.5.
|
|
|
Submission date |
Nov 18, 2013 |
Last update date |
Dec 29, 2014 |
Contact name |
Ming Tsao |
E-mail(s) |
[email protected]
|
Phone |
4169464501
|
Organization name |
University of Toronto
|
Department |
Ontario Cancer Institute
|
Lab |
Applied Molecular Oncology
|
Street address |
610 University Avenue
|
City |
Toronto |
State/province |
Ontario |
ZIP/Postal code |
M5G2M9 |
Country |
Canada |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE14814 |
Prognostic and Predictive Gene Signature for Adjuvant Chemotherapy in Resected Non-Small-Cell Lung Cancer |
|