|
| Status |
Public on Nov 02, 2009 |
| Title |
Arabidopsis plants treated with 1.6 mM Tween 20, collection time 2.5 h |
| Sample type |
RNA |
| |
|
| Source name |
leaf material from Arabidopsis thaliana plants
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Ecotype: Col-0
Age: 28 days
|
| Biomaterial provider |
Plant Physiology Department
|
| Treatment protocol |
The plants were sprayed with distilled water and the leave material was weighted and immediately frozen in liquid nitrogen. The harvesting time was 2.5 h after the treatment.
|
| Growth protocol |
For every experiment a total of three independent replicas (33 Arabidopsis thaliana Col-0 plants per experiment) were done. The plants used were growing under sterile conditions in 0.5 L glass jars containing MS medium (supplemented with vitamins and with 1% (w/v) sucrose and 0.8 % (w/v) agar). Growth occurred in a growth chamber with 8 h of light (150 ?E m-2 s-1 photosynthetically active radiation) and 16 h of darkness under controlled conditions (22?C to 24?C at day 18?C to 20?C at night and 70 to 90 % relative humidity).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from 1 g of leaf material was extracted with TRIzol? (Invitrogen, Karlsruhe, Germany). Three independently isolated RNA-samples were pooled for each experiment. RNA quality and quantity control, cRNA labeling, hybridizations, expression data normalization and analysis were performed in collaboration with Carsten M?and Thomas Altmann (Max-Planck-Institute for Molecular Plant Physiology, Golm) according to the Affymetrix manual and as described by M?et al. (2003).
|
| Label |
biotin
|
| |
|
| Description |
All the induction experiments in this study were performed at the same time of the day in the place were the plants were grown to avoid additional stresses and to eliminate any influence of circadian gene activity on results.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
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| |
|
| Submission date |
Aug 09, 2006 |
| Last update date |
Nov 02, 2009 |
| Contact name |
Gretel Mara Hunzicker |
| E-mail(s) |
[email protected]
|
| Organization name |
Ruhr-Universität
|
| Department |
Plant Physiology
|
| Lab |
Weiler
|
| Street address |
Universitätstr. 150
|
| City |
Bochum |
| State/province |
NRW |
| ZIP/Postal code |
44803 |
| Country |
Germany |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
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