|
| Status |
Public on Nov 12, 2013 |
| Title |
d-4 |
| Sample type |
RNA |
| |
|
| Source name |
opi10-_after heat shock
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
genotype/variation: opi10-
culture condition: cultured for 3 h at 30C after the heat shock
|
| Treatment protocol |
Shifted to 43C and cultured for 1 h, and then shifted back to 30C and cultured for 1 and 3 h.
|
| Growth protocol |
Cultured in liquid Edinburgh Minimal Medium at 30C to mid-log phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
phenol/chloroform
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using GeneChip 3'IVT Expression Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
cRNA were hybridized for 16 hr at 45C on GeneChip Yeast Genome 2.0 array in the GeneChip Hybridazation Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
SAMPLE 8
|
| Data processing |
Affymetrix default. ExpressionStat 5.0
|
| |
|
| Submission date |
Nov 11, 2013 |
| Last update date |
Nov 12, 2013 |
| Contact name |
Makoto Kimura |
| Organization name |
RIKEN
|
| Lab |
Cellular Dynamics Lab
|
| Street address |
2-1 Hirosawa
|
| City |
Wako |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE52259 |
Expression data from the Schizosaccharomyces pombe opi10- strain. |
|
