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Sample GSM1247494 Query DataSets for GSM1247494
Status Public on Jun 09, 2014
Title MO3.13 human oligodendroglioma cell line Replicate 2
Sample type RNA
 
Source name MO3.13 human oligodendroglioma cell line
Organism Homo sapiens
Characteristics cell line: MO3.13
cell type: oligodendroglioma cell line
treatment: control
Growth protocol MO3.13 cells were grown in DMEM containing 10 % fetal bovine serum (FBS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM of L-glutamine andSodium pyruvate. Total RNA was collected for microarray
Extracted molecule total RNA
Extraction protocol Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
Label biotin
Label protocol Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
 
Hybridization protocol cDNA was fragmented and 10ug hybridized to an Affymetrix Human Exon 1.0 ST Array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
Scan protocol Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
Description Gene expression data obtained from MO3.13 human oligodendroglioma cell line
Data processing RMA
 
Submission date Oct 22, 2013
Last update date Jun 09, 2014
Contact name Hu Li
E-mail(s) [email protected]
Organization name Mayo Clinic
Department Molecular Pharmacology & Experimental Therapeutics
Lab Systems Biology and Pharmacology
Street address 200 First Street, Gonda Building G19-408
City Rochester
State/province MN
ZIP/Postal code 55904
Country USA
 
Platform ID GPL5175
Series (1)
GSE51536 The Anti-Aging and Tumor Suppressor Protein Klotho Affects Signaling Pathwaysin a Human Oligodendroglioma Cell Line

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
3948543 6.95033
3063807 6.53669
3096575 6.04175
2949118 6.80101
3227645 8.78686
4014076 5.02386
2637819 2.14662
3850234 8.10803
3719161 9.6009
2916345 7.97393
2342904 5.47516
3817464 6.42563
2408437 6.3328
3932148 7.05037
3375091 5.75982
3653619 8.05033
2818035 4.48304
3063795 6.57384
2359282 8.75352
2981874 8.39769

Total number of rows: 22011

Table truncated, full table size 341 Kbytes.




Supplementary file Size Download File type/resource
GSM1247494_Control_2.CEL.gz 21.9 Mb (ftp)(http) CEL
GSM1247494_Control_2.rma-gene-core.chp.gz 176.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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