|
| Status |
Public on May 14, 2014 |
| Title |
1003-130004_Baseline_LS |
| Sample type |
RNA |
| |
|
| Source name |
skin biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
subject: 1003-130004
visit: Baseline
skin: LS
|
| Treatment protocol |
For each patient, one 4-mm skin punch biopsy was collected from lesion located on the trunk or extremities with approximately 0.5 mm or more of induration and approximately 5 mm from the edge of the plaque, and another from macroscopically normal non-lesion on a similar body area to the biopsy plaque (i.e. arm, arm or leg, leg). Biopsies were kept frozen –80oC until RNA preparation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Qiagen's RNeasy mini kit according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Target was prepared from 100ng total RNA using the NuGEN Ovation V2 kit, followed by NuGEN Encore biotin labeling module, using the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.875ug of cDNA was hybridized for 16 hr at 45oC on GeneChip HT HG-U133+ PM 96 array plates. GeneChips were washed and stained in the Affymetrix GeneChip Array Station.
|
| Scan protocol |
GeneChips were scanned using the GeneChipHT Scanner.
|
| Data processing |
The data was normalized by RMA using ArrayStudio version 6.
|
| |
|
| Submission date |
Oct 18, 2013 |
| Last update date |
May 14, 2014 |
| Contact name |
K Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Janssen R&D
|
| Street address |
1400 McKean Road
|
| City |
Spring House |
| State/province |
PA |
| ZIP/Postal code |
19477 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE51440 |
Guselkumab (interleukin-23-specific monoclonal antibody) demonstrates clinical and molecular response in moderate-to-severe psoriasis |
|
