|
| Status |
Public on Dec 01, 2013 |
| Title |
MH1C1_high_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MH1C1 cells exposed to 310 µM CoCl2 for 24 h
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: MH1C1
|
| Treatment protocol |
Exposures of 24 hours in duration were initiated once flasks were confluent using the test chemicals CoCl2.
|
| Growth protocol |
MH1C1 and H4-II-E-C3 cells (ATCC, Manassas, VA) were grown in Dulbecco's Modified Eagle's Medium (DMEM; Lonza, Walkersville, MD) containing 10% fetal bovine serum (Invitrogen, Carlsbad, CA) and 10 mL Glutamax (Invitrogen) in T75 flasks incubated at 37 °C with 5% carbon dioxide.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized on the GeneChip Rat Genome 230 2.0 Array using Affymetrix instrumentation according to the manufacturer’s instructions.
|
| Scan protocol |
Microarrays were scanned on an Affymetrix GeneChip Scanner following the manufacturer's instructions.
|
| Data processing |
Microarray data was processed for background adjustment, normalization, and summarization using the Robust Multi-Array Averaging method (RMA) using Partek Genomic Suite (GS) software (Version 6.6, St. Louis, MO).
|
| |
|
| Submission date |
Sep 26, 2013 |
| Last update date |
Dec 01, 2013 |
| Contact name |
Matthew Permenter |
| Organization name |
US Army Center for Environmental Health Research
|
| Street address |
568 Doughten Dr
|
| City |
Fort Detrick |
| State/province |
MD |
| ZIP/Postal code |
21758 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE51207 |
Exposure to Cobalt Causes Transcriptomic and Proteomic Changes in Two Rat Liver Derived Cell Lines |
|
