|
| Status |
Public on Jan 01, 2014 |
| Title |
H32_2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Hypopharyngeal squamous cell carcinoma No. 32
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Tumor tissue
age: 52
gender: Male
t: 4a
n: 1
m: 1
Stage: IVC
differentiation: moderate
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Hy3
|
| Label protocol |
We used miRCURY LNA microRNA Array Hi-Power Labeling kit.
|
| |
|
| Channel 2 |
| Source name |
H32 pooled sample
|
| Organism |
Homo sapiens |
| Characteristics |
pooled sample composition: blends equal amounts of each samples
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Hy5
|
| Label protocol |
We used miRCURY LNA microRNA Array Hi-Power Labeling kit.
|
| |
|
| |
| Hybridization protocol |
Denature the solution at 95 ℃ for 5 min. Centrifuge at 12,000 g at 4 ℃ for 2 min. Add 30 μL of 20 x salt Buffer to ditch of “Hybridization Chamber”. Slide is capped by “MASTUNAMI Gap Cover Glass (CG00044 or 24*60 25PCS)”. Fill the gap with 45 μL of the solution. Incubate in 56 ℃ water bath for 16-20 hr.
|
| Scan protocol |
Scanner; G2505C (Agilent), Software; Agilent Scan Control (Agilent), Green and Red PMT: 100 %, Scan resolution: 10 um
|
| Description |
CB-RNA76-7
|
| Data processing |
Quantification; Feature Extraction 10.7.3.1 (Agilent). Normalization; First, calculate the average ratio (Hy3/Hy5) of replicated spots on each array. Next, calculate the ratio of average ratio (Tumor/Non tumor).
|
| |
|
| Submission date |
Sep 24, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Naohiko Seki |
| Organization name |
Chiba University
|
| Street address |
Inohana1-8-1, Chuo-ku
|
| City |
Chiba city |
| State/province |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17728 |
| Series (1) |
| GSE51129 |
microRNA expression profile of hypopharyngeal squamous cell carcinoma |
|
