|
| Status |
Public on Aug 20, 2013 |
| Title |
VEC C |
| Sample type |
RNA |
| |
|
| Source name |
MycCap prostate cancer cells
|
| Organism |
Mus musculus |
| Characteristics |
batch: B
knock-in: VEC
cell line: MycCap prostate cancer cells
|
| Growth protocol |
after transfection and selection, cells were grown to confluence and harvested
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol RNA purification
|
| Label |
biotin
|
| Label protocol |
Affymetrix WT Labeling kit, according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Exon 1.0 arrays, according to manufacturer's instructions, washed with Fluidics 450
|
| Scan protocol |
Affymetrix M10 scanner, using software Affymetrix Genechip Command Console v2.0.0.1029
|
| Description |
total RNA from MyCaP culture
|
| Data processing |
All analysis performed in R. Data normalized using RMA from the limma package, and further normalization for Twist1-expression was carried out. For R script used for analysis, see supplemental data (Gajula et al., 2013)
|
| |
|
| Submission date |
Aug 19, 2013 |
| Last update date |
Aug 20, 2013 |
| Contact name |
Russell Williams |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University
|
| Department |
Radiation Oncology
|
| Lab |
Tran
|
| Street address |
1550 Orleans St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE50002 |
Effect of Twist-box mutation on gene expression induced by Twist1 overexpression |
|
