|
| Status |
Public on Jul 27, 2014 |
| Title |
H441_siTTF-1_TGF-beta_24h |
| Sample type |
RNA |
| |
|
| Source name |
siTTF-1 transfected H441 cells treated with TGF-beta for 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H441
tissue: human lung adenocarcinoma
|
| Treatment protocol |
Cells were transfected with siRNAs and stimulated with 3 ng/ml of TGF-beta for the indicated times.
|
| Growth protocol |
H441 cells were maintained in RPMI 1640 (#11875; Life Technologies) supplemented with 10% FBS, 100 U/ml penicillin G, and 100 ug/ml streptomycin. Cells were grown in a humidified atmosphere with 5% CO2 at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using RNeasy mini kit (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000).
|
| |
|
| Hybridization protocol |
Array hybridization was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
|
| Scan protocol |
Array scanning was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
|
| Data processing |
Microarray Suite software 5.0 (Affymetrix) was used to calculate the average difference (AD) for each gene probe set, which was shown as an intensity value of the gene expression (Ishii et al, Genomics 68:136-143, 2000).
|
| |
|
| Submission date |
Aug 08, 2013 |
| Last update date |
Jul 27, 2014 |
| Contact name |
Daizo Koinuma |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Tokyo
|
| Department |
Pathology
|
| Street address |
Hongo 7-3-1, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE49675 |
Expression data of human lung adenocarcinoma cell line H441 treated with TTF-1/NKX2-1 siRNA and TGF-beta |
| GSE51510 |
Role of TTF-1/NKX2-1, Smad3 and Smad4 on lung cancer cell lines |
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