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Sample GSM1204761 Query DataSets for GSM1204761
Status Public on Jul 27, 2014
Title H441_siTTF-1_TGF-beta_1p5h
Sample type RNA
 
Source name siTTF-1 transfected H441 cells treated with TGF-beta for 1.5h
Organism Homo sapiens
Characteristics cell line: H441
tissue: human lung adenocarcinoma
Treatment protocol Cells were transfected with siRNAs and stimulated with 3 ng/ml of TGF-beta for the indicated times.
Growth protocol H441 cells were maintained in RPMI 1640 (#11875; Life Technologies) supplemented with 10% FBS, 100 U/ml penicillin G, and 100 ug/ml streptomycin. Cells were grown in a humidified atmosphere with 5% CO2 at 37°C.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using RNeasy mini kit (QIAGEN).
Label biotin
Label protocol Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000).
 
Hybridization protocol Array hybridization was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
Scan protocol Array scanning was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
Data processing Microarray Suite software 5.0 (Affymetrix) was used to calculate the average difference (AD) for each gene probe set, which was shown as an intensity value of the gene expression (Ishii et al, Genomics 68:136-143, 2000).
 
Submission date Aug 08, 2013
Last update date Jul 27, 2014
Contact name Daizo Koinuma
E-mail(s) [email protected]
Organization name University of Tokyo
Department Pathology
Street address Hongo 7-3-1, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
 
Platform ID GPL570
Series (2)
GSE49675 Expression data of human lung adenocarcinoma cell line H441 treated with TTF-1/NKX2-1 siRNA and TGF-beta
GSE51510 Role of TTF-1/NKX2-1, Smad3 and Smad4 on lung cancer cell lines

Data table header descriptions
ID_REF
VALUE The AD values were normalized for each array so that the average of all AD values was 100.
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 232.1 P 0.000169
AFFX-BioB-M_at 357.3 P 0.000044
AFFX-BioB-3_at 228.6 P 0.00006
AFFX-BioC-5_at 646.3 P 0.000044
AFFX-BioC-3_at 798.3 P 0.000044
AFFX-BioDn-5_at 1265.1 P 0.000044
AFFX-BioDn-3_at 2723 P 0.00006
AFFX-CreX-5_at 6585.9 P 0.000052
AFFX-CreX-3_at 8101.7 P 0.000044
AFFX-DapX-5_at 2.6 A 0.737173
AFFX-DapX-M_at 6.1 A 0.514891
AFFX-DapX-3_at 1.1 A 0.969024
AFFX-LysX-5_at 7 A 0.250796
AFFX-LysX-M_at 2.3 A 0.659339
AFFX-LysX-3_at 1.9 A 0.440641
AFFX-PheX-5_at 4.1 A 0.81489
AFFX-PheX-M_at 0.9 A 0.883887
AFFX-PheX-3_at 7.4 A 0.327079
AFFX-ThrX-5_at 1.2 A 0.783496
AFFX-ThrX-M_at 7.7 A 0.262809

Total number of rows: 54675

Table truncated, full table size 1401 Kbytes.




Supplementary file Size Download File type/resource
GSM1204761_H441_siTTF_1p5h_133p2.CEL.gz 7.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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