|
| Status |
Public on Nov 04, 2014 |
| Title |
SMC ST1 -/- control, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
VSMCs, Stat1 KO, control
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: STAT1
genotype/variation: STAT1 knock-out
cell type: aortic vascular smooth muscle cells
treatment: control
|
| Treatment protocol |
VSMCS were treated with 10ng/ml IFNγ for 8h or with 1ug/ml of LPS for 4h or with IFNγ for 4h followed by IFNγ and LPS for additional 4h.
|
| Growth protocol |
VSMCs were cultivated in DMEM medium containing 4,5mg/l Glucose, 2mM L-glu, supplemented with 100U/ml penicillin and 100μg/ml streptomycin and 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from VSMCs using RNeasy Mini Kit (Qiagen) together with DNase digestion step according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays (LifeTechnologies).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
SAMPLE 15
8911191037_B
Cell line
Replicate 3
|
| Data processing |
Data were background subtracted and quantile normalised in GenomeStudio (Illumina).
|
| |
|
| Submission date |
Aug 02, 2013 |
| Last update date |
Nov 04, 2014 |
| Contact name |
Hans Bluyssen |
| E-mail(s) |
[email protected]
|
| Organization name |
Faculty of Biology, University of Adam Mickiewicz
|
| Department |
Department of Human Molecular Genetics
|
| Street address |
Umultowska 89
|
| City |
Poznań |
| ZIP/Postal code |
61-614 |
| Country |
Poland |
| |
|
| Platform ID |
GPL15097 |
| Series (1) |
| GSE49519 |
STAT1 and IRF8 orchestrate IFNγ and LPS-mediated signal integration in the vasculature that leads to amplified pro-atherogenic responses |
|
