|
| Status |
Public on Aug 05, 2013 |
| Title |
Spleen, control diet fed for 1day, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen, fed control diet for 1day
|
| Organism |
Mus musculus |
| Characteristics |
gender: Female
strain: BALB/c
age: 9 weeks
tissue: Spleen
diet: diet containing potato starch
|
| Treatment protocol |
Spleens were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Tokyo, Japan)..
|
| Label |
biotin
|
| Label protocol |
biotinylated aRNA was obtained from 100 ng of purified total RNA using a GeneChip 3’ IVT Express Kit (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
After fragmenting, 12.5 µg aRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip mouse genome 430 2.0 array (Affymetrix) GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
CEL files were quantified with the Robust Microarray Summarization (FARMS) algorithm, using the statistical language R and Bioconductor.
|
| |
|
| Submission date |
Aug 02, 2013 |
| Last update date |
Aug 05, 2013 |
| Contact name |
Yuichiro Fukui |
| E-mail(s) |
[email protected]
|
| Organization name |
Research Institute, Kagome Co., Ltd.
|
| Department |
Nature & Wellness Research Department
|
| Street address |
17 Nishitomiyama
|
| City |
Nasushiobara |
| State/province |
Tochigi |
| ZIP/Postal code |
329-2762 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE41127 |
Gene expression profile in the spleen of mice fed Lactobacillus brevis KB290 |
|
