|
| Status |
Public on Mar 07, 2014 |
| Title |
Muc1+_Pdpn+_E18-2 |
| Sample type |
RNA |
| |
|
| Source name |
Muc1+/Pdpn+ cells FACS purified from E18 lungs
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Lung
age: E18
cell population: Muc1+/Pdpn+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was performed using RNeasy Micro Kit from Qiagen according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified using the Two-Cycle cDNA synthesis kit from Affymetrix following manufacturer's instructions.
Biotinylated aRNA was prepared with the GeneChip 3' IVT Express Kit from Affymetrix following manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization of aRNA to Mouse Genome 430 2.0 Array from Affymetrix was achieved following manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
biological rep2
|
| Data processing |
The data were analyzed with Expression Console using Affymetrix default analysis settings (MAS 5.0).
|
| |
|
| Submission date |
Jul 30, 2013 |
| Last update date |
Mar 07, 2014 |
| Contact name |
Mark Krasnow |
| Organization name |
Stanford University
|
| Street address |
Beckman Center, B400 279 W. Campus Dr.
|
| City |
Stanford |
| State/province |
Ca |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE49346 |
Expression data from adult ATII and E18 Bipotent progenitor cells in the mouse lung |
|
