|
| Status |
Public on Jan 03, 2014 |
| Title |
LNCaP.FoxA1.5 |
| Sample type |
RNA |
| |
|
| Source name |
Prostate cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
overexpression vector: FOXA1
|
| Treatment protocol |
Cells were transfected with 3μM pcDNA3.1_GFP (Control) or 3μM pcDNA3.1_FoxA1 using Lipofectamine2000 (Invitrogen). Cells were harvested 48h later.
|
| Growth protocol |
Cells were grown in RPMI + 10% FBS and standard antibiotics in a 37oC incubator with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNEasy with on column DNaseI digestion according the the manufacturer's instructions.Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridisation procedure
|
| Scan protocol |
Standard Illumina scanning procedure
|
| Description |
FOXA1 overexpressing LNCaP prostate cancer cell line replicate 5
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jul 23, 2013 |
| Last update date |
Sep 14, 2015 |
| Contact name |
Chandra Chilamakuri |
| E-mail(s) |
[email protected]
|
| Organization name |
Cancer Research UK Cambridge Institute
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE49153 |
Effect of FOXA1 overexpression in prostate cancer |
|
