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| Status |
Public on Jun 12, 2016 |
| Title |
10 R Day 2, Rep 3 |
| Sample type |
SRA |
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| Source name |
10 R Day 2
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| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole body
radiation dose: 10 Roentgen
time post-irradiation (days): 2
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| Extracted molecule |
total RNA |
| Extraction protocol |
Flies were aged 2, 10 and 20 days after irradiation, then collected using light anesthesia and frozen using liquid nitrogen. mRNA was extracted from whole flies.
Illumina TruSeq kit
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
Sequences were aligned using Tophat version 2.0
Gene counts were calculated using bioconductor package easyRNASeq, with annotation coming from the bioconductor package biomaRt
Differentially expressed genes were determined using the bioconductor package DESeq
Genome_build: dm3
Supplementary_files_format_and_content: text file with normalized gene expression
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| Submission date |
Jun 17, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Michael Antosh |
| Organization name |
Michael Antosh
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| Street address |
Brown University Box 1843
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| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02912 |
| Country |
USA |
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| Platform ID |
GPL13304 |
| Series (1) |
| GSE47999 |
Drosophila melanogaster Show a Threshold Effect in Response to Radiation |
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| Relations |
| Reanalyzed by |
GSM3283894 |
| BioSample |
SAMN02205056 |
| SRA |
SRX306624 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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