|
| Status |
Public on Jul 01, 2006 |
| Title |
Twice weekly physical activity, cardiac tissue, 16 months old 3 |
| Sample type |
RNA |
| |
|
| Source name |
Heart left ventricle in female Sprague-Dawley rats after physical activity twice-weekly
|
| Organism |
Rattus norvegicus |
| Characteristics |
Gender: Female, Strain: Sprague-Dawley (Charles River)
Age: 16 months; Tissue: Heart left ventricle
|
| Treatment protocol |
Reference Schweitzer et al. Access to exercise and its relation to cardiovascular health and gene expression in laboratory animals. Life Sciences 77: 2246-2261, 2005.
|
| Growth protocol |
Reference Schweitzer et al. Access to exercise and its relation to cardiovascular health and gene expression in laboratory animals. Life Sciences 77: 2246-2261, 2005.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using a RNeasy Fibrous Tissue Mini kit (Qiagen) according to the manufacturer's recommendations.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
High integrity RNA was hybridized to the Affymetix GeneChip® Rat Genome 230 2.0 Array
|
| Scan protocol |
Arrays were scanned using the GeneArray scanner (Agilent Technologies)
|
| Description |
Gene expression data from female Sprague-Dawley rats that had atwice weekly 1 hr physical activity in a large box (PA)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) and RMA Express.
|
| |
|
| Submission date |
Jun 15, 2006 |
| Last update date |
Jun 15, 2006 |
| Contact name |
Natalie Schweitzer |
| E-mail(s) |
[email protected]
|
| Phone |
513-280-0190
|
| Fax |
513-529-5006
|
| Organization name |
Miami University
|
| Street address |
|
| City |
Oxford |
| State/province |
OH |
| ZIP/Postal code |
45056 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE5085 |
Expression data from female SD rats with access to lifelong exercise |
|
