|
| Status |
Public on Dec 31, 2013 |
| Title |
Male 8week AJ CBDLrep2 |
| Sample type |
RNA |
| |
|
| Source name |
AJCBDL
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: A/J
tissue: jejunum
gender: male
age: 8 weeks
treatment: cholestatic intestinal injury
|
| Treatment protocol |
Either CBDL surgery or sham surgery.
|
| Growth protocol |
7 days following CBDL or sham surgery, the animals were euthanized and the jejunum was harvested under sterile conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA performed per manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared per standard Affymetrix protocol from 1ug total RNA.
|
| |
|
| Hybridization protocol |
10ug of cRNA were hybridized for 16 hours at 45degrees on Mouse Gene 1.0 ST Arrays.
|
| Scan protocol |
Scanned on an Affymetrix scanner.
|
| Description |
Gene expression data from jejunum
|
| Data processing |
The data were normalized by Affymetrix Expression Console using RMA method.
|
| |
|
| Submission date |
May 20, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Susan G Dorsey |
| E-mail(s) |
[email protected]
|
| Phone |
410-706-7250
|
| Organization name |
University of Maryland Baltimore
|
| Department |
School of Nursing
|
| Street address |
655 West Lombard Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE47099 |
Genes involved in cholestatic intestinal injury |
|
