|
| Status |
Public on Dec 01, 2013 |
| Title |
germinal center B cells 5 |
| Sample type |
RNA |
| |
|
| Source name |
germinal center B cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: germinal center B cells
|
| Treatment protocol |
Cells were microdissected from HE stained kryosections
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was amplified using the One-direct-Kit from NUGEN applied for 250 microdissected cells
|
| Label |
biotin
|
| Label protocol |
Samples were labelled with Encore biotin module (NUGEN) washed and stained using the HWS Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Human Gene 1.0 ST arrays (Affymetrix) were hybridized with 5.5 mg labelled cDNA.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
CD77_GCB_TE5
|
| Data processing |
Statistical analysis was done with the statistical computing environment R. Additional software packages (affy, geneplotter, multtest, and vsn) were taken from the Bioconductor project. Microarray preprocessing: Probe level normalization was conducted using the variance stabilization method (vsn) from Huber et al..
probe group file: unsupported Human Gene 1.0 ST Array CDF Files
meta-probeset file: HuGene-1_0-st-v1 Probeset Annotations, CSV Format, Release 30 (11 MB 01/22/10)
|
| |
|
| Submission date |
May 17, 2013 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Claudia Döring |
| E-mail(s) |
[email protected]
|
| Organization name |
Johann Wolfgang-Goethe University Frankfurt a.M.
|
| Department |
Medical School, Institite of Pathology
|
| Street address |
Theodor Stern Kai 7
|
| City |
Frankfurt |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE47044 |
Nodular lymphocyte predominant hodgkin lymphoma and T cell/histiocyte rich large B cell lymphoma - endpoints of a spectrum of one disease? |
|
| Relations |
| Reanalyzed by |
GSE86357 |
