|
| Status |
Public on Jul 24, 2013 |
| Title |
Wild-type retina, biological rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Wild-type retina at P15
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype: Homozygous Wild-type
developmental stage: P15
|
| Growth protocol |
Dmbo mice were backcrossed with C57BL/6JWT to remove the Rd1 mutation. Wild-type and dumbo mice were maintained in a 12-hours light/12-hours dark cycle with free access to food and water. Animals were killed at P15 by cervical dislocation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Retinas were isolated under a microscope to remove extra retinal tissue and snap-frozen at -80°C. Total RNA was individually isolated from each whole retina and purified using the RNeasy minikit as described by the manufacturer. RNA quantities were assessed by NanoDrop®ND-1000 spectrophotometer
|
| Label |
biotin
|
| Label protocol |
For each sample, 100ng of total RNA were amplified using the WT sense strand Target Labelling kit (Affimetryx). The resulting sense cDNA was fragmented by UDG and APE 1 and biotin-labelled with TdT using the GeneChip® WT Terminal labelling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Gene 1.0 ST arrays were hybridized according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol FS450_0007).
|
| Scan protocol |
The arrays were scanned using the GeneChip® Scanner 3000 7G (Affymetrix) and raw data were extracted from the scanned images and analyzed with the Affymetrix Power Tools software package.
|
| Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA normalization method.
|
| |
|
| Submission date |
May 16, 2013 |
| Last update date |
Jul 24, 2013 |
| Contact name |
Arnaud Boulling |
| E-mail(s) |
[email protected]
|
| Phone |
+4127 205 79 00
|
| Organization name |
Institute for Research in Ophthalmology
|
| Street address |
Avenue de Grand-Champsec 64
|
| City |
SION |
| ZIP/Postal code |
1950 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE47002 |
Transcriptome comparison of murine wild-type and dumbo retinas at P15 |
|
