|
Status |
Public on Nov 24, 2014 |
Title |
110440_Macrophage_PolyICH |
Sample type |
RNA |
|
|
Source name |
Macrophage, asymptomatic, polyICH
|
Organism |
Homo sapiens |
Characteristics |
cell type: macrophages treatment: PolyICH age: 59 gender: female batch: 2 phenotype: asymptomatic
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from PBMC and macrophage samples was extracted using the RNeasy mini kit (Qiagen, CA).
|
Label |
biotin
|
Label protocol |
Preparation of cDNA, cRNA, and labeling were carried out by the Yale Center for Genomic Analysis using the standard Illumina protocols.
|
|
|
Hybridization protocol |
Hybridization buffer from the BeadChip kit (Illumina) was mixed with 1500 ng of biotin-labeled cRNA, heated to 65°C for 5 minutes, and then loaded onto the BeadChip. The BeadChips were sealed in a hybridization chamber and placed in an oven at 58°C with a rocker for 16-20 hours. After the hybridization, the BeadChips were washed and stained in a series of washes and stains as outlined in the Illumina protocol.
|
Scan protocol |
The BeadChips were scanned on the Illumina Iscan.
|
Data processing |
The data were normalised using quantile normalisation with lumi in R.
|
|
|
Submission date |
May 06, 2013 |
Last update date |
Nov 24, 2014 |
Contact name |
Hailong Meng |
Organization name |
Yale University
|
Street address |
300 George Str
|
City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06525 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE46681 |
Profiling the successful response to infection with West Nile virus |
|