|
| Status |
Public on Jun 04, 2013 |
| Title |
WT replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
WT MEFs
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6 and 129 mixed
genotype/variation: wild type
cell type: Primary mouse embryonic fibroblasts (MEFs)
|
| Treatment protocol |
Cre was introduced into the cells via retroviral infection and selected in media containing 2.5ug/ml puromycin.
|
| Growth protocol |
Primary mouse embryonic fibroblasts were harvested from E13.5 embryos and maintained in DMEM containing 10% FBS, 1% NEAA, 2mm glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's directions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
According to the manufacturer’s instructions
|
| Scan protocol |
GeneChip arrays were scanned according Affymetrix standard protocol using Scanner type M10
|
| Description |
MEFS_WT-3
|
| Data processing |
MAS 5.0 algorithm
|
| |
|
| Submission date |
May 03, 2013 |
| Last update date |
Jul 19, 2013 |
| Contact name |
Peter J Park |
| E-mail(s) |
[email protected]
|
| Phone |
617-432-7373
|
| Organization name |
Harvard Medical School
|
| Department |
Center for Biomedical Informatics
|
| Street address |
10 Shattuck St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE46645 |
The Swi/Snf tumor suppressor complex establishes nucleosome occupancy at target promoters [expression] |
|
