|
| Status |
Public on Apr 25, 2013 |
| Title |
clock mutant mouse uninfect night biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
clock mutant mouse uninfect night cecal tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Clock mutant
infection: uninfected
circadian time: night (10pm)
age: 8 - 14 weeks
tissue: cecal mucosa
|
| Treatment protocol |
Mice were treated with streptomycin intragastrically 24 hours before inoculation with S. Typhimurium. At 72 hours after infection, mice were euthanized
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard protocols described in the Affymetrix Gene Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from clock mutant mouse uninfect night cecal tissue
12_CK_UN_3
|
| Data processing |
The data were analyzed with BRB Array Tools version 4.2.1
|
| |
|
| Submission date |
Apr 24, 2013 |
| Last update date |
Apr 25, 2013 |
| Contact name |
Michael George |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California, Davis
|
| Department |
Medical Microbiology and Immunology
|
| Street address |
451 Health Sciences Drive
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE46356 |
Expression data from mouse cecum |
|
