|
| Status |
Public on Aug 08, 2013 |
| Title |
ESCs-overexp-5 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129/Sv
cell type: Embryonic stem cells
genotype: Prdm4-EGFP
|
| Growth protocol |
Embryonic stem cells were cultured according to standard protocols
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled according to the standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Prdm4-EGFP overexpressor replicate 5
|
| Data processing |
Rank invariant normalization with background subtraction using Gene Expression Module V1.9.0 of Illumina Studio
|
| |
|
| Submission date |
Apr 23, 2013 |
| Last update date |
Aug 08, 2013 |
| Contact name |
Andrew Christopher Nelson |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Warwick
|
| Department |
School of Life Sciences
|
| Street address |
Gibbet Hill Campus
|
| City |
Coventry |
| ZIP/Postal code |
CV4 7AL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE46308 |
Expression analysis of Prdm4 mutant and overexpressing mouse embryonic stem cells |
|
