|
| Status |
Public on Apr 04, 2013 |
| Title |
p65 KO mouse KLSF #3 |
| Sample type |
RNA |
| |
|
| Source name |
p65 KO_KLSF
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: p65 knockout
cell type: bone marrow lineage-Flk2-c-kit+Sca-1+ cells
|
| Growth protocol |
Murine bone marrow lineage-Flk2-Sca-1+c-kit+ cells were sorted directly into buffer RLT (Qiagen) with 1% β-Mercaptoethanol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the RNEasy Mini Kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
RNA was amplified and labeled using the Nugen Ovation Pico WTA System V2.
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix Mouse Gene Chip 1.0 ST arrays and scanned at the UNC Affymetrix Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix).
|
| Description |
SAMPLE 6
|
| Data processing |
RMA expression value derived from Expression Console software, and analysis was performed using Partek Genomics Suite.
affymetrix-algorithm-name = rma-gene-default, affymetrix-algorithm-version = 1.0, program-name = Expression Console, program-version = 1.1.2800.28061
|
| |
|
| Submission date |
Apr 03, 2013 |
| Last update date |
Apr 04, 2013 |
| Contact name |
Sarah Stein |
| E-mail(s) |
[email protected]
|
| Phone |
919-966-3884
|
| Fax |
919-966-8212
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Lineberger Comprehensive Cancer Center
|
| Lab |
Baldwin
|
| Street address |
450 West Dr.
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE45755 |
Deletion of the NF-κB subunit p65/RelA in the hematopoietic compartment leads to defects in hematopoietic stem cell function. |
|
