|
| Status |
Public on Nov 12, 2013 |
| Title |
BreastAdjacentNormal-S427-Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Adjacent Normal Breast tissue from patient 427
|
| Organism |
Homo sapiens |
| Characteristics |
patient id: patient 427
tissue type: Adjacent Normal
er: NA
pr: NA
her2: NA
tnm: NA
grade: NA
Stage: NA
|
| Growth protocol |
The tissue are collected from patients directly.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For RNA extraction, total RNA was extracted from tissues collected from the patients by Trizol® Reagent (Invitrogen, USA) according to the instruction mannual. RNA purified was quantified at OD260nm by using a ND-1000 spectrophotometer (Nanodrop Technology, USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 nano labchip kit (Agilent Technologies, USA).
|
| Label |
Cy3
|
| Label protocol |
After RNA extraction, 100 ng of total RNAs were dephosphorylated and labeled with pCp-Cy3 by Agilent miRNA Complete Labeling and Hyb Kit (Agilent Technologies, USA, microRNA Spike- In Apply).
|
| |
|
| Hybridization protocol |
2X hybridization buffer (Agilent Technologies, USA) was added to the labeled mixture to a final volume of 45ul. The mixture was heated for 5 min at 100°C and immediately cooled to 0°C. Each 45ul sample was hybridized onto an Agilent human miRNA Microarray Release 12.0, 8x15K (Agilent Technologies, USA) at 55°C for 20 hours.
|
| Scan protocol |
Slides were scanned on an Agilent microarray scanner (Agilent Technologies, model G2505C) at 100% and 5% sensitivity settings.
|
| Description |
S427N1
Adjacent breast normal sample obtained from patient
|
| Data processing |
Feature Extraction (Agilent Technologies) software version 10.7.3.1 was used for image analysis.
|
| |
|
| Submission date |
Apr 01, 2013 |
| Last update date |
Nov 12, 2013 |
| Contact name |
Chia-Hsien Lee |
| E-mail(s) |
[email protected]
|
| Organization name |
National Taiwan University
|
| Street address |
No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan(R.O.C)
|
| City |
Taipei |
| ZIP/Postal code |
10617 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL14767 |
| Series (1) |
| GSE45666 |
MicroRNA expression profile of breast cancer patients with adjacent normal control group. |
|
