|
| Status |
Public on May 24, 2006 |
| Title |
wt embryonic humeri at day E14.5, biological rep1 (B) |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryos at day E14.5
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: Runx2 wildtype
Age: embryonic stage E14.5
|
| Treatment protocol |
humeri were disected in RNAlater (Qiagen) and stored in liquid nitrogen until RNA isolation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
peqGOLD TriFast (peqLab) extraction of total RNA was performed according to the manufacturer's instructions followed by Qiagen RNeasy column purification including an on column Dnase digestion according to manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
10 µg of total RNA were first reverse transcribed into cDNA (cDNA Synthesis System, Roche). The cDNA was used for in vitro transcription (T7 Megascript Kit, Ambion) and labeling with Biotin-11-CTP and Biotin-16-UTP (Perkin-Elmer, Boston, USA)
|
| |
|
| Hybridization protocol |
biotin-labeled cRNA was hybridized at a concentration of 60 ng/µl for 16 hr at 45C on Affymetrix GeneChip Murine Genome U74 Version 2 Set MG-U74A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner G2500A (Agilent Technologies)
|
| Description |
Gene expression data from Runx2 wt mouse embryonic humeri.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 23, 2006 |
| Last update date |
May 23, 2006 |
| Contact name |
Jochen Hecht |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Mundlos
|
| Street address |
Ihnestr. 73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL82 |
| Series (1) |
| GSE4911 |
Expression data from mouse E14.5 wt and RUNX2 -/- humeri |
|
