|
| Status |
Public on Dec 31, 2015 |
| Title |
C_spleen_5d_24h_rep4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
pooled whole mouse tissue cell line
|
| Organism |
Mus musculus |
| Characteristics |
sample type: Strategen universal reference RNA (Cat. No. 740100)
|
| Treatment protocol |
C57B6 mice were exposed to SJL aggressor mice for periods of 5 days and 10days (6 hours each day) to induce anxiety/stress which parallels to PTSD in human. Organs, blood and brain regions were collected after 24 hours and 1.5 week of post 5 days social defeat period; and 24 hour and 6 weeks post 10 days social stress period.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol from brain regions and organs and using PAXgene from blood following the manufacturers' instructions
|
| Label |
Cy3
|
| Label protocol |
RNA was reverse transcribed and labeled using Agilent's Mouse Chip Labeling and Detection Kit (Agilent Technologies, Inc.) following the manufacturer’s protocol.
|
| |
|
| Channel 2 |
| Source name |
C_spleen_5d_24h
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57B/6
sample type: control (C)
tissue: spleen
|
| Treatment protocol |
C57B6 mice were exposed to SJL aggressor mice for periods of 5 days and 10days (6 hours each day) to induce anxiety/stress which parallels to PTSD in human. Organs, blood and brain regions were collected after 24 hours and 1.5 week of post 5 days social defeat period; and 24 hour and 6 weeks post 10 days social stress period.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol from brain regions and organs and using PAXgene from blood following the manufacturers' instructions
|
| Label |
Cy5
|
| Label protocol |
RNA was reverse transcribed and labeled using Agilent's Mouse Chip Labeling and Detection Kit (Agilent Technologies, Inc.) following the manufacturer’s protocol.
|
| |
|
| |
| Hybridization protocol |
The slides were hybridized at 60ºC for 16 h
|
| Scan protocol |
Hybridized slides were scanned and recorded using a Agilent's Feature Extraction Software v 10.9 (Agilent Technology Inc.)
|
| Description |
C_spleen_5d_24h_rep4
US09493743_251486832510_1_3 - C5-130 5day 24hrs control.txt
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent's Feature Extraction Software V10.9 was used.
|
| |
|
| Submission date |
Mar 12, 2013 |
| Last update date |
Dec 31, 2015 |
| Contact name |
seid muhie |
| E-mail(s) |
[email protected]
|
| Organization name |
USACEHR
|
| Department |
Systems Biology Enterprise
|
| Street address |
568 Doughten Dr
|
| City |
Fort Detrick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (2) |
| GSE68076 |
Transcriptome profiling of spleen, blood and hemi-brain of social stressed C57B6 mice exhibiting PTSD like features |
| GSE68077 |
Transcriptome characterization of social stressed C57B6 mice exhibiting PTSD like features |
|
