|
| Status |
Public on Feb 15, 2013 |
| Title |
Uninfected spleen, day 0, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
uninfected_spleen harvested on day 0
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6
age: 6-10 weeks
infected with: none (uninfected control)
tissue: spleen
harvest time point: day 0
|
| Treatment protocol |
Mice were infected intravenously via the retro-orbital sinus with 2x10^6 plaque forming unites of LCMV-Armstrong or LCMV-Clone 13.
|
| Growth protocol |
Six-ten week old mice were used for all experiments, and were housed under pathogen-free conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Harvested spleens were frozen in RNA Later (Qiagen) at 1 mg/ml tissue. Whole spleens were homogenized, and RNA from homogenates were isolated with Rneasy extraction kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Ambion WT labeling kit
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Genes ST 1.0 microarray
|
| Scan protocol |
Affymetrix Expression Console
|
| Description |
N3A
Gene expression data from spleens harvested at day 0, from uninfected C57BL7 mice aged 6-10 weeks
|
| Data processing |
Expression values were subjected to RMA normalization using Affymetrix CDF probeset definition and GeneSpringGX v7.3.1.
Relative expression levels for each probeset were calculated by normalization to the median value for all uninfected samples
|
| |
|
| Submission date |
Feb 14, 2013 |
| Last update date |
Feb 15, 2013 |
| Contact name |
Rebekah Karns |
| E-mail(s) |
[email protected]
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Gastroenterology, Hepatology, & Nutrition
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45276 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE44322 |
Gene expression profile analysis of mouse whole spleen following infection by lymphocytic choriomeningitis virus (LCMV), comparing LCMV-Armstrong and LCMV-Clone 13 expression patterns at 0, 5, 9, and 30 days |
|
