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Sample GSM1070613 Query DataSets for GSM1070613
Status Public on Jan 26, 2013
Title RMG1 - proliferating-rep3
Sample type RNA
 
Source name RMG1 - proliferating
Organism Homo sapiens
Characteristics cell type: Exponentially proliferating ovarian cancer cells
cell line: RMG1
Treatment protocol No treatment
Growth protocol Ovarian cancer cell lines were plated and harvested when 50-80% confluent. RNA was extracted and hybridized on Affymetrix microarrays. At least 3 biological repeats were performed for each cell line.
Extracted molecule total RNA
Extraction protocol QIAgen Rneasy was used to extract RNA
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
 
Hybridization protocol Hybridization was for 16hrs andaccording to the standard Affymetrix protocol
Scan protocol GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450 using standard Fluidics Protocol ((Fluidics protocol FS450_007)). GeneChips were scanned using the Affymetrix 7G Scanner
Data processing The data was gc-rma normalised using the R Bioconductor package affy.
 
Submission date Jan 25, 2013
Last update date Sep 01, 2016
Contact name Karen Elizabeth Sheppard
E-mail(s) [email protected]
Phone 61 3 9656 3758
Fax 61 3 96563738
Organization name Perter MacCallum Cancer Centre
Department Research
Street address St Andrews Place
City Melbourne
State/province Victoria
ZIP/Postal code 3008
Country Australia
 
Platform ID GPL6244
Series (1)
GSE43765 Expression data from exponentially proliferating ovarian cancer cell lines
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Log base 2 gcrma normalised expression value

Data table
ID_REF VALUE
7892501 8.315940019
7892502 5.089510772
7892503 3.424315617
7892504 10.04970426
7892505 3.115291204
7892506 4.882865779
7892507 4.954334112
7892508 5.642107052
7892509 12.96802826
7892510 2.731784213
7892511 3.255877896
7892512 7.894987853
7892513 3.877312769
7892514 11.51218066
7892515 8.69614106
7892516 6.875758817
7892517 4.931858437
7892518 3.185277608
7892519 5.286183691
7892520 9.580424115

Total number of rows: 33252

Table truncated, full table size 645 Kbytes.




Supplementary file Size Download File type/resource
GSM1070613_3_RMG1.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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