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Sample GSM1070570 Query DataSets for GSM1070570
Status Public on Jan 26, 2013
Title JHOC.9 - proliferating-rep3
Sample type RNA
 
Source name JHOC.9 - proliferating
Organism Homo sapiens
Characteristics cell type: Exponentially proliferating ovarian cancer cells
cell line: JHOC.9
Treatment protocol No treatment
Growth protocol Ovarian cancer cell lines were plated and harvested when 50-80% confluent. RNA was extracted and hybridized on Affymetrix microarrays. At least 3 biological repeats were performed for each cell line.
Extracted molecule total RNA
Extraction protocol QIAgen Rneasy was used to extract RNA
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
 
Hybridization protocol Hybridization was for 16hrs andaccording to the standard Affymetrix protocol
Scan protocol GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450 using standard Fluidics Protocol ((Fluidics protocol FS450_007)). GeneChips were scanned using the Affymetrix 7G Scanner
Data processing The data was gc-rma normalised using the R Bioconductor package affy.
 
Submission date Jan 25, 2013
Last update date Sep 01, 2016
Contact name Karen Elizabeth Sheppard
E-mail(s) [email protected]
Phone 61 3 9656 3758
Fax 61 3 96563738
Organization name Perter MacCallum Cancer Centre
Department Research
Street address St Andrews Place
City Melbourne
State/province Victoria
ZIP/Postal code 3008
Country Australia
 
Platform ID GPL6244
Series (1)
GSE43765 Expression data from exponentially proliferating ovarian cancer cell lines
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Log base 2 gcrma normalised expression value

Data table
ID_REF VALUE
7892501 5.765014894
7892502 4.220195774
7892503 3.197643249
7892504 9.852871583
7892505 3.218125214
7892506 4.006029989
7892507 4.418520594
7892508 3.535163424
7892509 12.66410847
7892510 3.83432594
7892511 3.146891409
7892512 8.191985366
7892513 3.415652834
7892514 11.34447519
7892515 8.514747904
7892516 5.576695688
7892517 5.290104021
7892518 3.223518065
7892519 5.702747688
7892520 9.741283336

Total number of rows: 33252

Table truncated, full table size 645 Kbytes.




Supplementary file Size Download File type/resource
GSM1070570_3_JHOC-9.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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