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Sample GSM1070543 Query DataSets for GSM1070543
Status Public on Jan 26, 2013
Title COLO.704 - proliferating-rep3
Sample type RNA
 
Source name COLO.704 - proliferating
Organism Homo sapiens
Characteristics cell type: Exponentially proliferating ovarian cancer cells
cell line: COLO.704
Treatment protocol No treatment
Growth protocol Ovarian cancer cell lines were plated and harvested when 50-80% confluent. RNA was extracted and hybridized on Affymetrix microarrays. At least 3 biological repeats were performed for each cell line.
Extracted molecule total RNA
Extraction protocol QIAgen Rneasy was used to extract RNA
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
 
Hybridization protocol Hybridization was for 16hrs andaccording to the standard Affymetrix protocol
Scan protocol GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450 using standard Fluidics Protocol ((Fluidics protocol FS450_007)). GeneChips were scanned using the Affymetrix 7G Scanner
Data processing The data was gc-rma normalised using the R Bioconductor package affy.
 
Submission date Jan 25, 2013
Last update date Sep 01, 2016
Contact name Karen Elizabeth Sheppard
E-mail(s) [email protected]
Phone 61 3 9656 3758
Fax 61 3 96563738
Organization name Perter MacCallum Cancer Centre
Department Research
Street address St Andrews Place
City Melbourne
State/province Victoria
ZIP/Postal code 3008
Country Australia
 
Platform ID GPL6244
Series (1)
GSE43765 Expression data from exponentially proliferating ovarian cancer cell lines
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Log base 2 gcrma normalised expression value

Data table
ID_REF VALUE
7892501 6.640033269
7892502 5.436050657
7892503 4.284143644
7892504 10.15097263
7892505 2.923520417
7892506 3.920153829
7892507 5.178826664
7892508 5.542892641
7892509 12.92697187
7892510 4.492251991
7892511 3.867016298
7892512 7.691801651
7892513 3.494082163
7892514 11.1713427
7892515 9.034668254
7892516 5.842210727
7892517 6.912245703
7892518 3.234715662
7892519 5.19293564
7892520 9.212492597

Total number of rows: 33252

Table truncated, full table size 645 Kbytes.




Supplementary file Size Download File type/resource
GSM1070543_3_COLO-704.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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